Food Science

Annual reports on fermentation processes Volume 6 - download pdf or read online

By George T Tsao; Michael C Flickinger; Robert K Finn

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Tolbert Joseph Feder Monsanto Company St. Louis, Missouri I. INTRODUCTION Large-scale growth of vertebrate cells in vitro offers a method for the production of biomolecules not available from other sources. This has focused the attention of researchers on the development of mass culture systems which offer opportunities for large-scale growth of such cells for production of desired biomolecules. Although the recent explosion of recombinant DNA methodology offers an approach for the production of biomolecules derived from these cell types using conventional large-scale microbial fermentation, this new technology is useful only after the identification and the availability of a specific protein and its gene.

The continuous perfusion of fresh medium at a rate proportional to the cell density roughly stabilized both nutrient and by-product concentration. 9 x 10 9 cells/ml. 6-fold increase in the cell yield over the conventional system. Table 2 shows the results of several 4 and 40-liter runs with this system. Table 2. 66 harvest Tolbert al. 54 WILLIAM R. TOLBERT AND JOSEPH FEDER The last entry in Table 2 describes use of a 40-liter perfusion reactor in a chemostat mode (Figure 8 ) . The reactor was operated as above until a cell density of 9 x 10 6 cells/ml was obtained.

Suspended cell-free media may be further processed for isolation of secreted products. All Figure 3. 100-Liter vibromixer agitated culture reactor is shown in warm room during growth stage (a) and during harvest with Western States STMIOOO centrifuge (b). Agitator disks and other internal parts are shown in (c). , (32) 48 WILLIAM R. TOLBERT AND JOSEPH FEDER Table 1. 3° Rat Osteosarcoma Medulloblastoma Careinosarcoma Cells grew as small suspension. aggregate clumps or as single cells b in b Representative yields cells from at least Corresponds Reprinted to ca.

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